Role of ferritinophagy in cystine deprivation-induced cell death in glioblastoma cells

Ferroptosis is a form of cell death caused by iron-dependent lipid peroxidation. Cancer cells increase cystine uptake for the synthesis glutathione (GSH), which used peroxidase 4 to reduce peroxides. Here, we report that deprivation in glioblastoma cells, but not inhibition GSH l-buthionine sulfoximine (BSO), induces ferroptosis. We found decreased protein levels ferritin heavy chain FTH1, whereas it was increased BSO treatment. The lysosome inhibitor bafilomycin A1 or deletion nuclear receptor coactivator (NCOA4) inhibited deprivation-induced decrease FTH1 and death. In addition, induced microtubule-associated light 3 (LC3)-II accumulation, suggesting ferritinophagy. causes when are treated with iron inducers, ferrous ammonium sulfate hemin. On other hand, degradation required glutamine. This study suggests ferritinophagy, addition depletion, plays an important role ferroptosis cells.